Purification and Concentration of Antigens for Complement Fixation by Methods of Dialysis, Adsorption, and Extraction

Isolation and purification of Echinococcus granulosus antigen B from hydatid cyst fluid using three different methods

Hydatid cyst, the larval stage of cestodes Echinococcus spp., is recognized as a zoonotic infection in the world. The World Health Organization (WHO) has recently classified echinococcosis in a group of neglected tropical diseases.The prevalence of Echinococcus granulosus infection is high in Iran due to the presence of various intermediate hosts in this country. Considering the rising trend of...

تشخیص آنتی ژن های توموری در عقده لمفاوی گاو مبتلا به لنفوسارکوم ویروسی با روش رسوب ایمنی

In this study One lymph node from cow with viral lymphosarcoma and one lymph node from healthy cow were analyzed by three different methods for protein extraction, include simple homogenizing in PBS, sonication and phenol extraction. Tumor antigen detection relies on immunoprecipitation followed by SDS-PAGE. In this experiment Streptococcus pyogenes was used for purification of immune compl...

A Novel Efficient Method for Extraction and Purification of Recombinant Phenylalanine Dehydrogenase

Growing applications of phenylalanine dehydrogenase (PheDH) enzyme in the medical and pharmaceutical industries encourages researchers to seek simple, fast and economical alternative purification methods. With goal of finding a new technique, the extraction and purification of recombinant Bacillus badius PheDH in polyethylene glycol 6000 (PEG-6000) and ammonium sulfate aqueous two-phase systems...

Design and construction of a helium purification system using cryogenic adsorption process

One of the most appropriate methods for elimination of trace impurities in helium is cryogenic adsorption process. So, in this study design and construction of cryogenic adsorption helium purification system (3 Nm3.hr-1, 80 bar) carried out using activated carbon as adsorbent at 77K. To evaluation of adsorption dynamics and effect of pressure on elimination of trace impurities, helium purificat...

Detection of rotavirus by Latex Agglutination Test (Rotales); Comparison with Electron Microscopy and Complement Fixation Test